Our primary objective is to conduct a comprehensive investigation into the presence and extent of insecticide resistance within P. xylostella populations in the Rio Grande Valley (RGV). To achieve this, we gather baseline data on the susceptibility to three commonly used active ingredients with distinct modes of action: Chlorantraniliprole (Coragen), Spinetoram (Radiant), and Emamectin (Proclaim). By evaluating the response of diamondback moths to these insecticides, we can assess the level of resistance and potential cross-resistance among different chemical classes. The data collected will serve as a valuable resource for updating management recommendations specifically tailored to vegetable production in RGV and other vegetable-producing regions in Texas. By understanding the resistance patterns and dynamics, we will develop effective insecticide rotation strategies to mitigate the risk of resistance development and optimize pest control in agricultural systems. These recommendations will assist farmers and agricultural professionals in making informed decisions regarding insecticide selection and application, thereby enhancing the sustainability and productivity of vegetable crops in the region. Furthermore, monitoring the trends and changes in insecticide resistance over time is crucial for proactive pest management and the preservation of effective chemical control options. By regularly assessing insecticide resistance levels in diamondback moth populations, we can detect emerging resistance mechanisms, track the effectiveness of different insecticides, and guide the adjustment of management strategies as needed.