Breeding for spinach improvement requires new methods to speed up the development of cultivars with increased yield potential and disease and pest resistance. In Texas, the most yield-limiting diseases in spinach production include White Rust (WR) and recently Anthracnose, caused by Albugo occidentalis and Colletotrichum dematium, respectively. Since natural infection levels in the field varies year-to-year, conventional selection of resistant cultivars in the field is time consuming and unreliable. Therefore, in order to improve cultivar-development efficiency, the breeding program is evaluating spinach-breeding lines for disease resistance to develop molecular markers linked to resistance traits.